We have now available a modified expression vector for SUMO3 fusion proteins in pETM11 with an optimized synthetic sumo3 gene with appropiate restriction sites at the 3'-end of the gene for sumo3 gene for insertion of any gene of interest. The unique feature of this protease is the recognition of the 3-dimensional structure of SUMO3 unlike other proteases that recognize specific amino acid sequences.
Thus, unspecific proteolysis of your protein by SenP2 does not occur. Your target gene can be inserted via the Bam HI- site and one of the restriction sites downstream of it. This Ser will be left in your protein after cleavage with SenP2.
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In any case, you have to make sure that you have the terminal two Glycine codons of SUMO3 in-frame with your target gene. Note : The first amino acid after the Glycines must not be Proline!
How to clone you gene into the SUMO vectors
The protease cannot cleave the protein in that case! Yunus and Christopher D. Tatham and Ronald T. Leach, Xufan Tian, Michael R.
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- A. Solutions and Reagents.
- Proteome-wide identification of SUMO modification sites by mass spectrometry.
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SUMO: Methods and Protocols by Manuel S. Rodriguez - olagynulehyb.gq
Temporarily Out of Stock Online Please check back later for updated availability. Overview While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. Product Details Table of Contents. Table of Contents Part I.
- Anti-Sumo 2 + Sumo 3 antibody [SM23/] (ab) | Abcam.
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