Get e-book SUMO Protocols

Free download. Book file PDF easily for everyone and every device. You can download and read online SUMO Protocols file PDF Book only if you are registered here. And also you can download or read online all Book PDF file that related with SUMO Protocols book. Happy reading SUMO Protocols Bookeveryone. Download file Free Book PDF SUMO Protocols at Complete PDF Library. This Book have some digital formats such us :paperbook, ebook, kindle, epub, fb2 and another formats. Here is The CompletePDF Book Library. It's free to register here to get Book file PDF SUMO Protocols Pocket Guide.

SUMOylation kit

We have now available a modified expression vector for SUMO3 fusion proteins in pETM11 with an optimized synthetic sumo3 gene with appropiate restriction sites at the 3'-end of the gene for sumo3 gene for insertion of any gene of interest. The unique feature of this protease is the recognition of the 3-dimensional structure of SUMO3 unlike other proteases that recognize specific amino acid sequences.

Thus, unspecific proteolysis of your protein by SenP2 does not occur. Your target gene can be inserted via the Bam HI- site and one of the restriction sites downstream of it. This Ser will be left in your protein after cleavage with SenP2.

  • Globalization and Informal Jobs in Developing Countries - A Joint Study of the International Labour Office and the Secretariat of the World Trade Organization.
  • Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins.
  • Recommended for you.
  • Time-dependent Partial Differential Equations and Their Numerical Solution!
  • From Basic Research to Nanotechnology Carbon Nanotubes.

In any case, you have to make sure that you have the terminal two Glycine codons of SUMO3 in-frame with your target gene. Note : The first amino acid after the Glycines must not be Proline!

How to clone you gene into the SUMO vectors

The protease cannot cleave the protein in that case! Yunus and Christopher D. Tatham and Ronald T. Leach, Xufan Tian, Michael R.

Sumo (Simulation of Urban Mobility) Tutorial Part 1: Manually Creation of Network

See All Customer Reviews. Shop Textbooks.

  1. Nel mio cor sempre contrasta;
  2. A. Solutions and Reagents.
  3. Proteome-wide identification of SUMO modification sites by mass spectrometry.
  5. Add to Wishlist. USD Sign in to Purchase Instantly.

    SUMO: Methods and Protocols by Manuel S. Rodriguez -

    Temporarily Out of Stock Online Please check back later for updated availability. Overview While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. Product Details Table of Contents. Table of Contents Part I.

    • Anti-Sumo 2 + Sumo 3 antibody [SM23/] (ab) | Abcam.
    • Introduction.
    • Pattern Discovery in Bioinformatics: Theory & Algorithms (Chapman & Hall Crc Mathematical & Computational Biology Series).
    • Purification Protocols.
    • Jesus: Uncovering the Life, Teachings, and Relevance of a Religious Revolutionary.
    • Computers Helping People with Special Needs: 9th International Conference, ICCHP 2004, Paris, France, July 7-9, 2004. Proceedings;
    • Khi to Freedom?

    Show More. Average Review.