e-book Cardiomyocytes: Methods and Protocols

Free download. Book file PDF easily for everyone and every device. You can download and read online Cardiomyocytes: Methods and Protocols file PDF Book only if you are registered here. And also you can download or read online all Book PDF file that related with Cardiomyocytes: Methods and Protocols book. Happy reading Cardiomyocytes: Methods and Protocols Bookeveryone. Download file Free Book PDF Cardiomyocytes: Methods and Protocols at Complete PDF Library. This Book have some digital formats such us :paperbook, ebook, kindle, epub, fb2 and another formats. Here is The CompletePDF Book Library. It's free to register here to get Book file PDF Cardiomyocytes: Methods and Protocols Pocket Guide.

Heart J. Nunes, S. Biowire: a platform for maturation of human pluripotent stem cell-derived cardiomyocytes.

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes | Protocol

Methods 10 , — Thavandiran, N. Design and formulation of functional pluripotent stem cell-derived cardiac microtissues. USA , E— Turnbull, I. Advancing functional engineered cardiac tissues toward a preclinical model of human myocardium. Hinson, J. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy. Kuppusamy, K. Let-7 family of microRNA is required for maturation and adult-like metabolism in stem cell-derived cardiomyocytes. Stillitano, F. Genomic correction of familial cardiomyopathy in human engineered cardiac tissues. Riegler, J. Human engineered heart muscles engraft and survive long term in a rodent myocardial infarction model.

Zhang, D. Tissue-engineered cardiac patch for advanced functional maturation of human ESC-derived cardiomyocytes. Biomaterials 34 , — Wang, G.

How to purify cardiomyocytes

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies. Eschenhagen, T. Three-dimensional reconstitution of embryonic cardiomyocytes in a collagen matrix: a new heart muscle model system. Zimmermann, W. Three-dimensional engineered heart tissue from neonatal rat cardiac myocytes. Tissue engineering of a differentiated cardiac muscle construct. Neuber, C. Stohr, A. Contractile abnormalities and altered drug response in engineered heart tissue from Mybpc3-targeted knock-in mice.

Dussurget, O. Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera.

Cardiomyocyte apoptosis in heart development: methods and protocols.

Ungrin, M. Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One 3 , e Insulin inhibits cardiac mesoderm, not mesendoderm, formation during cardiac differentiation of human pluripotent stem cells and modulation of canonical Wnt signaling can rescue this inhibition. Stem Cells 31 , — Tran, T. Wnt3a-induced mesoderm formation and cardiomyogenesis in human embryonic stem cells.

Freund, C. Insulin redirects differentiation from cardiogenic mesoderm and endoderm to neuroectoderm in differentiating human embryonic stem cells. Stem Cells 26 , — Patient-specific induced pluripotent stem-cell models for long-QT syndrome. Takahashi, K. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell , — Physiological aspects of cardiac tissue engineering. Heart Circ. Lotz, S. Sustained levels of FGF2 maintain undifferentiated stem cell cultures with biweekly feeding. PLoS One 8 , e Download references. We thank A. Moretti and K.

Correspondence to Arne Hansen. To obtain permission to re-use content from this article visit RightsLink. Advanced Drug Delivery Reviews Circulation International Journal of Molecular Sciences Cell Stem Cell Nature Protocols By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Article metrics. Advanced search.

Skip to main content. You are viewing this page in draft mode.

Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes

Subjects Cardiovascular models Cell culture Induced pluripotent stem cells Stem-cell differentiation Tissue engineering. Abstract Since the advent of the generation of human induced pluripotent stem cells hiPSCs , numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and then subsequently assess their ability to recapitulate the properties of adult human cardiomyocytes. Rent or Buy article Get time limited or full article access on ReadCube. References 1. PubMed Article Google Scholar 7. Article Google Scholar 8. PubMed Article Google Scholar 9. PubMed Article Google Scholar Google Scholar Acknowledgements We thank A.

Integrated supplementary information Supplementary figures 1. Supplementary information PDF files 1. Supplementary Figures Supplementary Figures 1 and 2. Rights and permissions To obtain permission to re-use content from this article visit RightsLink. About this article Publication history Published 11 May Wang , Robert D. Sampson , Sunthar Kanayaganam , George E. Taffet , Lloyd H. Michael , Mark L. Harding , Caroline M. Wobma , Alessandro Vasciaveo , Edward C.

Comments By submitting a comment you agree to abide by our Terms and Community Guidelines. Article Tools. Nature Protocols menu. Nature Research menu. Search Article search Search. See our User Agreement and Privacy Policy. See our Privacy Policy and User Agreement for details. Published on Nov 1, In this educational webinar, Dr. Ronglih Liao and Dr.

Subscribe to our newsletter

Davor Pavlovic discuss best practices for isolating healthy, calcium-tolerant adult cardiomyocytes for functional studies. They describe their techniques, the basic principles and theory behind those methodologies and modifications that should be considered based on an experiment's species and reagents. Specifically, Dr. Liao presents a proven, Langendorff-based method and discusses how to evaluate the success of an isolation based on calcium tolerance, morphology and functional outputs.

Following, Dr.

Pavlovic reviews an innovative Langendorff-free method for myocyte isolation, comparing and contrasting it with the traditional procedure and discussing the pros and cons of each. This is an essential webinar for basic researchers utilizing isolated cardiac myocytes, those who have considered using them but have found the isolation procedure too daunting, and those who wish to improve their technique to produce more meaningful, reproducible data.

SlideShare Explore Search You. Submit Search. Successfully reported this slideshow. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads.


  • Historic Floors: Their History and Conservation (Butterworth-Heinemann Series in Conservation and Museology)!
  • Cardiomyocytes.
  • Josephine Baker (Black Americans of Achievement).

You can change your ad preferences anytime. Techniques and Best Practices for Cardiomyocyte Isolation. Upcoming SlideShare.

Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: revolutionising research

Like this presentation? Why not share! Embed Size px. Start on. Show related SlideShares at end. WordPress Shortcode. InsideScientific Follow. Published in: Science. Full Name Comment goes here. Are you sure you want to Yes No. Lilian Vaughn who will win this game? No Downloads.

Product details

Views Total views. Actions Shares. Embeds 0 No embeds. No notes for slide. Techniques and Best Practices for Cardiomyocyte Isolation 1. Ronglih Liao and Davor Pavlovic discuss techniques for isolating myocytes using both traditional and Langendorff-free methodologies, including key considerations, best practices and how to achieve and confirm optimal myocyte quality Techniques and Best Practices for Cardiomyocyte Isolation LifeScienceWebinar ISCxIonOptix 2.

InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools and laboratory services 4. Liao and InsideScientific. All Rights Reserved rliao7 stanford. Using fine-tipped forceps, grip the sides of the aorta… Now slide the aorta over the cannula.


  • Science of Swimming Faster?
  • Recommended for you.
  • Stages of Thought: The Co-Evolution of Religious Thought and Science;
  • Primary cells and human embryonic stem cell sources?

Use the clip to hold the aorta in place.