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Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes | Protocol
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Correspondence to Arne Hansen. To obtain permission to re-use content from this article visit RightsLink. Advanced Drug Delivery Reviews Circulation International Journal of Molecular Sciences Cell Stem Cell Nature Protocols By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Article metrics. Advanced search.
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Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes
Subjects Cardiovascular models Cell culture Induced pluripotent stem cells Stem-cell differentiation Tissue engineering. Abstract Since the advent of the generation of human induced pluripotent stem cells hiPSCs , numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and then subsequently assess their ability to recapitulate the properties of adult human cardiomyocytes. Rent or Buy article Get time limited or full article access on ReadCube. References 1. PubMed Article Google Scholar 7. Article Google Scholar 8. PubMed Article Google Scholar 9. PubMed Article Google Scholar Google Scholar Acknowledgements We thank A.
Integrated supplementary information Supplementary figures 1. Supplementary information PDF files 1. Supplementary Figures Supplementary Figures 1 and 2. Rights and permissions To obtain permission to re-use content from this article visit RightsLink. About this article Publication history Published 11 May Wang , Robert D. Sampson , Sunthar Kanayaganam , George E. Taffet , Lloyd H. Michael , Mark L. Harding , Caroline M. Wobma , Alessandro Vasciaveo , Edward C.
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Davor Pavlovic discuss best practices for isolating healthy, calcium-tolerant adult cardiomyocytes for functional studies. They describe their techniques, the basic principles and theory behind those methodologies and modifications that should be considered based on an experiment's species and reagents. Specifically, Dr. Liao presents a proven, Langendorff-based method and discusses how to evaluate the success of an isolation based on calcium tolerance, morphology and functional outputs.
Pavlovic reviews an innovative Langendorff-free method for myocyte isolation, comparing and contrasting it with the traditional procedure and discussing the pros and cons of each. This is an essential webinar for basic researchers utilizing isolated cardiac myocytes, those who have considered using them but have found the isolation procedure too daunting, and those who wish to improve their technique to produce more meaningful, reproducible data.
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Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: revolutionising research
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Views Total views. Actions Shares. Embeds 0 No embeds. No notes for slide. Techniques and Best Practices for Cardiomyocyte Isolation 1. Ronglih Liao and Davor Pavlovic discuss techniques for isolating myocytes using both traditional and Langendorff-free methodologies, including key considerations, best practices and how to achieve and confirm optimal myocyte quality Techniques and Best Practices for Cardiomyocyte Isolation LifeScienceWebinar ISCxIonOptix 2.
InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools and laboratory services 4. Liao and InsideScientific. All Rights Reserved rliao7 stanford. Using fine-tipped forceps, grip the sides of the aorta… Now slide the aorta over the cannula.
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Use the clip to hold the aorta in place.